Search results for " STEM"
showing 10 items of 2170 documents
SARS-CoV-2 vaccine response and rate of breakthrough infection in patients with hematological disorders
2022
Abstract Background The clinical efficacy of SARS-CoV-2 vaccines according to antibody response in immunosuppressed patients such as hematological patients has not yet been established. Patients and methods A prospective multicenter registry-based cohort study conducted from December 2020 to December 2021 by the Spanish transplant and cell therapy group was used to analyze the relationship of antibody response at 3–6 weeks after full vaccination (2 doses) with breakthrough SARS-CoV-2 infection in 1394 patients with hematological disorders. Results At a median follow-up of 165 days after complete immunization, 37 out of 1394 (2.6%) developed breakthrough SARS-CoV-2 infection at median of 77 …
Design and experimental validation of a magnetic device for stem cell culture.
2020
Cell culture of bone and tendon tissues requires mechanical stimulation of the cells in order to mimic their physiological state. In the present work, a device has been conceived and developed to generate a controlled magnetic field with a homogeneous gradient in the working space. The design requirement was to maximize the magnetic flux gradient, assuring a minimum magnetizing value in a 15 mm × 15 mm working area, which highly increases the normal operating range of this sort of devices. The objective is to use the machine for two types of biological tests: magnetic irradiation of biological samples and force generation on paramagnetic particles embedded in scaffolds for cell culture. The…
Improvement of osteogenic differentiation of human mesenchymal stem cells on composite poly l-lactic acid/nano-hydroxyapatite scaffolds for bone defe…
2020
Tissue engineering offers new approaches to repair bone defects, which cannot be repaired physiologically, developing scaffolds that mimic bone tissue architecture. Furthermore, biomechanical stimulation induced by bioreactor, provides biomechanical cues that regulate a wide range of cellular events especially required for cellular differentiation and function. The improvement of human mesenchymal stem cells (hMSCs) colonization in poly-L-lactic-acid (PLLA)/nano- hydroxyapatite (nHA) composite scaffold was evaluated in terms of cell proliferation (dsDNA content), bone differen- tiation (gene expression and protein synthesis) and ultrastructural analysis by comparing static (s3D) and dynamic…
Transcriptomic responses to biotic stresses in Malus x domestica: a meta-analysis study.
2017
AbstractRNA-Seq analysis is a strong tool to gain insight into the molecular responses to biotic stresses in plants. The objective of this work is to identify specific and common molecular responses between different transcriptomic data related to fungi, virus and bacteria attacks in Malus x domestica. We analyzed seven transcriptomic datasets in Malus x domestica divided in responses to fungal pathogens, virus (Apple Stem Grooving Virus) and bacteria (Erwinia amylovora). Data were dissected using an integrated approach of pathway- and gene- set enrichment analysis, Mapman visualization tool, gene ontology analysis and inferred protein-protein interaction network. Our meta-analysis revealed…
Evolving by deleting: patterns of molecular evolution of Apple stem pitting virus isolates from Poland
2019
In this study, 267 coat protein gene (CP) sequences from 48 Polish isolates of Apple stem pitting virus (ASPV) were determined. The genetic structure of the virus population was analysed and possible mechanisms of molecular evolution explored. We found evidence of recombination within the ASPV population and the presence of 17 ASPV molecular variants that differ in the length, number and arrangement of deletions in the CP. Population genetic analyses showed significant variation among isolates from pear and apple trees, between isolates from the same host species and, more interestingly, within isolates, supporting the existence of significant levels of variability within individual hosts, …
Water recycling in leaves of Lithops (Aizoaceae)
2016
Lithops plants consist of a pair of succulent leaves inserted on a short stem; each growing season young leaves develop in a cavity formed between the older pair. Young leaves can take up water from the older pair allowing the plant to maintain growth and leaf expansion even without external supply of water. Recycling water between vegetative organs is one of the possible adaptation strategies of plants under drought stress, but it had never been demonstrated experimentally in Lithops. The methodology used to verify the existence of water redistribution from old leaves to young leaves was fluorescence microscopy, using two dyes to follow the water pathway inside the plant: Sulforhodamine G …
Soil and Regulated Deficit Irrigation Affect Growth, Yield and Quality of ‘Nero d’Avola’ Grapes in a Semi-Arid Environment
2021
The present work studied the effect of two consecutive years of regulated deficit irrigation (RDI) compared to rain fed management on the vegetative growth, yield, and quality of ‘Nero d’Avola’ grapes. The trial was conducted separately in two soils (vertisol and entisol) located at the top and bottom hillside of the same vineyard. Vertisol was characterized by greater depth, organic matter, exchangeable K20, and total N than entisol. RDI was based on an irrigation volume at 25% of estimated crop evapotranspiration (ETc) up to end of veraison and 10% of estimated ETc up to 15 days before harvest. Predawn water potential (PDWP) was used as indicator of plant water status and irrigation timin…
Major trends in stem anatomy and growth forms in the perianth-bearing Piperales, with special focus on Aristolochia.
2014
International audience; BACKGROUND AND AIMS: The order Piperales has the highest diversity of growth forms among the earliest angiosperm lineages, including trees, shrubs, climbers and herbs. However, within the perianth-bearing Piperales (Asarum, Saruma, Lactoris, Hydnora, Prosopanche, Thottea and Aristolochia), climbing species only occur in the most species-rich genus Aristolochia. This study traces anatomical and morphological traits among these lineages, to detect trends in growth form evolution and developmental processes. METHODS: Transverse stem sections of different developmental stages of representatives of Asarum, Saruma, Lactoris, Hydnora, Thottea and Aristolochia were compared …
Seasonal effects on mortality rates and resprouting of stems treated with glyphosate in the invasive tree of heaven (Ailanthus altissima (Mill.) Swin…
2015
Tree of heaven (Ailanthus altissima) is regarded as invasive within urban and natural areas worldwide. Efficient methods to control it are significantly needed if we are to limit its well-known environmental and economic impacts. Up to now the use of herbicides has proven necessary since following mechanical damage, Ailanthus vigorously resprouts. However, the seasonal response of Ailanthus stems, treated with herbicides, has never been assessed. We compared the control efficacy recorded in autumn, winter and summer in an abandoned suburban citrus grove in Sicily, under Mediterranean-climate conditions. Glyphosate was injected within drill holes made in knee-high cut trees. Tree mortality a…
Decoupling of light intensity effects on the growth and development of C3 and C4 weed species through sucrose supplementation
2002
Light availability has a profound effect on plant growth and development. One of the ways to study the effects of light intensity on plant growth and development without the confounding problem of photosynthate availability is sucrose injection/supplementation. A greenhouse experiment was conducted to evaluate the effects of light levels (0% and 75% shade) and sucrose injection (distilled water or 150 g sucrose l(-1)) on three weed species: redroot pigweed (Amaranthus retroflexus L., C4), lambsquarters (Chenopodium album L., C3) and velvetleaf (Abutilon theophrasti Medic., C3). The average total sucrose uptake was 7.6 and 5.9 g per plant for 0% and 75% shading, respectively, representing 47…